5 Tips about attune flow cytometer You Can Use Today
5 Tips about attune flow cytometer You Can Use Today
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Data-enriched characterization of mobile populations. Morphological info from images can insert towards the richness of flow cytometry details. As an example, the figure exhibits an usually regular apoptosis assay utilizing Annexin V and PI, introducing cell imaging to characterize cells in Just about every populace to reveal morphologically distinctive attributes. These insights couldn't have been received from multiplex staining on your own.
As samples are acquired about the Attune CytPix Flow Cytometer, the substantial-pace brightfield digital camera captures and outlets pictures of detected events in a fee up to 6,000 photos for every second, with regards to the flow fee and image dimensions. For increased versatility, Attune Cytometric Software lets you regulate the impression capture frequency as essential and choose a selected gate for graphic capture, if demanded.
Merge acoustic focusing technological innovation with higher-parameter analysis for flexible spectral unmixing and traditional workflows.
Explore new insights into your experimental knowledge by visualizing populations employing blended fluorescence and morphology info. Our application interface wizards permit downsampling, concatenation (merged data files) and dimensionality tools which can be created in to the software program for an intuitive, streamlined data Assessment working experience.
"An image just isn't rather worth a thousand dots, however it truly really helps to see illustrations or photos. It is great to see illustrations or photos that correlate with staining. By way of example, AnnexinV-good cells which might be morphologically different than wholesome cells, or CD14+ monocytes that happen to be much larger and even more textured than CD3+/CD4+/CD14- lymphocytes.
On top of that, it can be illuminating to determine the quantity of doublets slip as a result of standard singlet gates, and excellent to validate the status of sudden double-positive situations in my analysis."
Optimizing gating method. Even strong guide singlet gating is mistake-vulnerable and stays a subjective decision position in Nearly all flow cytometry assays. Imaging can be used to verify and regulate gates to include only single cells of desire.
Our experts will style and design panels for you personally as often times you'll need—cost-free, no product acquire required.
Early indications of this contamination experienced to begin with been dismissed as debris. Backgating to discover new populations. Backgating imaged cells about the Attune CytPix Flow Cytometer also enables you to use morphological options to find out fascinating subpopulations that might not be obvious from flow cytometry facts on your own.
End users can look at function images in serious-time to confirm sample good quality, improve protocols, and report the morphology of each and every cell inhabitants identified for Evaluation. Imaging seize also Gains from acoustic concentrating, which really helps to position the cells to ensure a pointy, centered graphic is attained.
Audio waves help specific mobile alignment, resulting in Outstanding data top quality and ~10X more rapidly sample flow prices.
Application Observe: Automated impression Investigation cuts down consumer-to-user variability in flow cytometry gating procedures
Constructed-in dimensionality reduction applications greater resolution into populations. Fluorescently gated populations again-gated onto UMAP plot to verify anticipated population resolution in lysed whole human blood. here Person can see occasions from Just about every cluster for Visible affirmation of cell phenotypes.
Versatile payment plan from Agilent Economical Options enable you to get the significant instrumentation you may need for quick project, or to develop your lab.
At the guts of each Attune Flow Cytometer lies our revolutionary acoustic concentrating know-how, which utilizes seem waves to meticulously align cells inside the flow cell. This progressive method surpasses common hydrodynamic techniques, leading to a lot quicker processing speeds, Extraordinary knowledge high quality, and greater sample throughput.
The Attune Xenith cytometer is usually a superior-parameter spectral analyzer, providing both of those spectral unmixing and conventional flow capabilities for extensive and multipurpose cell Investigation.
Agilent Benefit Guarantee ensures you at the very least a decade of instrument use from the date you purchase. Otherwise, We are going to credit you With all the residual value of the program toward an upgraded product.
What seemed to be a homogeneous population is actually much more assorted—an Perception that ought to be viewed as when interpreting effects.